作为细胞特异性aptamer结合的分子点的CD71的解和结构建模
Xiaoqiu Wu1, Honglin Liu1,2, Dongmei Han1
1Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province , Hunan University , Changsha , Hunan 410082 , China.
研究人员确定CD71是aptamerXQ-2d的点,对于胰腺癌的诊断和治疗至关重要. 这种aptamer具有针对性药物输送的潜力,改善了癌症治疗结果.
科学领域:
- 生物技术
- 分子生物学
- 癌症研究
背景情况:
- 胰腺癌是一种致命的疾病,
- 此前已经开发了一种ssDNA向体,XQ-2d,以向胰腺癌.
- 确定XQ-2d的特定结合点对于其治疗应用至关重要.
研究的目的:
- 为了识别ssDNA的目标 XQ-2d.
- 描述XQ-2d及其目标之间的结合相互作用.
- 开发基于XQ-2d的瘤向治疗方法.
主要方法:
- 细胞-SELEX 策略开发 XQ-2d 体.
- CD71 敲击实验以确认目标结合.
- 与转移素和抗CD71抗体进行竞争性结合测定.
- 用于测量结合点距离的表面能量转移纳米控制器.
- 用于结合相互作用分析的分子动力学模拟.
- 用于药物输送的XQ-2d-doxorubicin复合物的工程.
主要成果:
- CD71被确定为 XQ- 2d 的特定结合标.
- XQ-2d与膜结合的CD71结合,并与转移素共享结合部位.
- XQ-2d与抗CD71抗体结合点之间的距离大约为15纳米.
- 分子动力学模拟阐明了XQ-2d和转移素与CD71的竞争性结合机制.
- 一个基于XQ-2d的药物输送系统成功设计.
结论:
- 在胰腺癌中,CD71是被验证的 XQ- 2d 受体的标.
- XQ-2d作为诊断生物标志物和治疗剂具有潜力.
- 开发的基于XQ-2d的诊断工具为癌症治疗提供了一种新方法.
更多相关视频
10:46A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
Published on: September 13, 2022
12:31A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
相关概念视频
Structure of Benzene: Molecular Orbital Model
Molecular Models
Molecular Structure and Acidity
The size effect explains the change in atomic size on acidity. When comparing the acids formed from elements that belong to the same column in the periodic table, their atomic sizes...
Acid Strength and Molecular Structure
In the absence of any leveling effect, the acid strength of binary compounds of hydrogen with nonmetals (A) increases as the H-A bond strength decreases down a group in the periodic table. For group 17, the order of increasing acidity is HF < HCl < HBr < HI. Likewise, for group 16, the order of increasing acid strength is H2O < H2S < H2Se < H2Te. Across a row in the periodic table, the acid strength of binary hydrogen compounds increases with increasing...
Lewis Structures of Molecular Compounds and Polyatomic Ions
Conserved Binding Sites
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
