哺乳动物胚胎大小和细胞命运的液压控制
Chii Jou Chan1, Maria Costanzo2, Teresa Ruiz-Herrero3
1European Molecular Biology Laboratory, Heidelberg, Germany. cchan@embl.de.
Nature
|June 14, 2019
概括
胚胎大小由内部流体压力和组织机制控制. 增加的压力会导致生长, 但过多会导致细胞破裂,
科学领域:
- 发育生物学
- 生物物理
- 细胞生物学
背景情况:
- 组织发育和平衡依赖于精确的尺寸控制.
- 虽然研究了细胞增殖,但胚胎大小控制及其对细胞命运的影响仍然不清楚.
研究的目的:
- 研究充满液体的光在小鼠胚芽细胞大小控制和细胞命运规范中的作用.
- 了解连接光压,组织力学和胚胎发育的机制.
主要方法:
- 使用小鼠囊作为模型系统.
- 使用生物物理,胚胎,药物和遗传乱技术.
- 开发了一种液压关闭振荡的理论,以建模胚胎大小动态.
主要成果:
- 在胚胎细胞发育过程中,光膜压力增加两倍与皮质皮质张力增加和组织硬度相关.
- 增加皮层张力促进了素机械感知和紧密结合成熟,为光线生长创造了反循环.
- 超过关键的皮质张力值会导致肌体破裂和胚囊塌.
- 干扰紧密结节或增加组织硬导致胚胎尺寸较小.
- 灯膜压力和尺寸会影响 trofhectoderm 细胞分裂模式,影响细胞分配和命运.
结论:
- 发光压力和组织力学是组织尺度上的胚胎大小的关键调节者.
- 这些机械线索与细胞的位置和命运有关.
- 这项研究揭示了控制胚胎大小和细胞命运的新型液压动机制.
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