一个bHLH转录因子的突变允许杏仁化
R Sánchez-Pérez1,2,3, S Pavan4,5, R Mazzeo2,3,6
1Department of Plant Breeding, CEBAS-CSIC, Campus Universitario de Espinardo, 30100 Espinardo, Spain. rsanchez@cebas.csic.es stefano.pavan@uniba.it.
Science (New York, N.Y.)
|June 15, 2019
概括
杏仁的化包括通过鉴定基因突变来选择甜蜜的核. 这种bHLH2转录因子的突变阻止了杏仁的产生,杏仁是一种野生杏仁中存在的有毒化合物.
科学领域:
- 植物遗传学
- 农业科学
- 生物化学
背景情况:
- 野生杏仁种类含有有毒的杏仁素,
- 杏仁的化是通过选择无毒的,甜蜜的核子来实现的.
研究的目的:
- 为了确定杏仁中甜心核的遗传基础.
- 了解桃体生物合成及其调节的分子机制.
主要方法:
- 杏仁基因组的基因组测序和组装.
- 基于地图的克隆利用F1种群进行核味分离.
- 转录因子和相关基因的功能性表征.
主要成果:
- 鉴定了一组46基基因集群,其中包含五种基本的螺旋环-螺旋环 (bHLH) 转录因子 (bHLH1-bHLH5).
- bHLH2转录因子调节桃体生物合成基因 (PdCYP79D16和PdCYP71AN24).
- 在bHLH2中发生的特定点突变会破坏这些基因的转录,导致甜蜜的核.
结论:
- 杏仁中的甜心核特征是由bHLH2转录因子的突变控制的.
- 这种bHLH2的遗传变异是杏仁化过程中杏仁素产量丧失的原因.
- 了解这种途径可以了解作物改善和植物二次代谢物的演变.
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