细菌中的病毒体沿线贩运
Vorrapon Chaikeeratisak1, Kanika Khanna2, Katrina T Nguyen2
1Division of Biological Sciences, University of California, San Diego, San Diego, CA 92093, USA; Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Cell
|June 15, 2019
概括
细菌病毒使用动态细胞骨 (PhuZ) 来进行货物运输. 体沿着PhuZ纤维线移动到菌体核进行DNA包装,证明了细菌细胞骨的进化能力.
科学领域:
- 微生物学
- 结构生物学
- 分子生物学
背景情况:
- 细胞病毒使用基于微管的货物运输.
- 此前没有报告过这种机制的细菌实例.
- 大型菌体具有以素为基础的 (PhuZ) DNA 组织螺旋.
研究的目的:
- 研究 Pseudomonas 菌体中体运输和 DNA 包装的机制.
- 阐明PhuZ细胞骨在细菌病毒复制中的作用.
- 在细菌细胞骨架中演变的货物运输能力.
主要方法:
- 在膜上观察状体组合和沿着PhuZ纤维的运动.
- 对菌体核旋转和囊分布的分析.
- 研究PhuZ导线动力学,包括跑步机和GTP水解.
- 在GTP水解中缺陷的突变PhuZ纤维上研究状体的行为.
主要成果:
- 体聚集在膜上,并迅速沿着PhuZ细丝运输到菌体核.
- PhuZ螺旋旋转细胞核,促进囊的分布.
- 丝聚合驱动体运动和核旋转.
- 体在静态的PhuZ突变体上被固定,这表明了线程动态的重要性.
结论:
- 菌体被通过一个涉及极点的PhuZ聚合的机制运输.
- 这表明细菌细胞骨已经进化了货物运输功能.
- 基于PhuZ的传输对这些细菌的DNA包装和病毒复制至关重要.
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