在半合成生物中优化复制,转录和翻译
Aaron W Feldman1, Vivian T Dien1, Rebekah J Karadeema1
1Department of Chemistry , The Scripps Research Institute , 10550 North Torrey Pines Road , La Jolla , California 92037 , United States.
Journal of the American Chemical Society
|June 27, 2019
概括
这项研究优化了一种半合成生物 (SSO),以有效地使用DNA,RNA和翻译中的非正规氨基酸 (ncAAs) 产生蛋白质.
科学领域:
- 合成生物学
- 分子生物学
- 生物化学
背景情况:
- 半合成生物 (SSO) 可以使用非自然基对 (UBP) 存储增加的遗传信息.
- 之前的工作建立了能够与UBPs进行DNA复制,转录和翻译的SSO,产生具有非正规氨基酸 (ncAAs) 的蛋白质.
研究的目的:
- 系统地优化SSO以加强信息存储和检索.
- 为DNA复制,转录和翻译确定最佳的非自然核酸类型.
- 为了使多个近接的ncAAs有效地产生蛋白质.
主要方法:
- 探索不同类型的脱氧核酸和核酸类似物.
- 对非天然核糖三酸盐的体内结构活性关系 (SAR) 分析.
- 对非自然核酸的DNA和RNA聚合酶识别的评估.
- 评估蛋白质合成中非自然核酸的核酶介导配对.
主要成果:
- 确定了复制和转录的最佳UBP.
- 确定转录和翻译的最佳非天然核糖三酸盐.
- 在非自然核酸的聚合酶识别中观察到相似之处和差异.
- 在翻译过程中证明了各种非自然核酸的有效和选择性配对.
- 在优化的SSO中实现了多个近接ncAAs的高效蛋白质生产.
结论:
- 优化的SSO有效地整合了DNA,RNA和蛋白质合成中的非自然核酸.
- 这一进步显著扩大了SSO使用ncAAs创建新型蛋白质的能力.
- 这些发现为设计和设计SSO的先进生物应用提供了坚实的框架.
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