哺乳动物器官和物种之间 lncRNA 的发育动态
Ioannis Sarropoulos1, Ray Marin2, Margarida Cardoso-Moreira3,4
1Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
Nature
|June 28, 2019
概括
研究人员在七种物种的器官发育过程中探索了长非编码RNA (lncRNA). 他们发现许多lncRNA是特定于物种的,并具有动态表达,这表明它们在发育过程中起着至关重要的作用.
科学领域:
- 基因组学
- 发育生物学
- 进化生物学
背景情况:
- 长非编码RNAs (lncRNAs) 是丰富的,但在功能上缺乏特征.
- 它们在物种间的器官发育中的作用在很大程度上是未知的.
研究的目的:
- 在器官发育过程中系统分析 lncRNA 表达模式.
- 确定参与哺乳动物器官生成和进化的候选 lncRNA.
主要方法:
- 在七种物种 (人类, rhesus macaque,小鼠,大鼠,子,) 中对 lncRNA 表达的比较分析.
- 在七个主要器官的多个发育时间点表达.
- 特定物种和保存的 lncRNAs 的识别和描述.
主要成果:
- 每个物种确定了15,00035,000个候选 lncRNA,具有高度的物种特异性.
- 在多个发育阶段观察到 lncRNA 的动态表达模式.
- 发现从保存到血统和器官特异性的 lncRNAs 的发育转变.
结论:
- 在器官发育过程中, lncRNAs表现出动态和特定物种的表达.
- 这些发现为研究器官生成中的 lncRNA 功能提供了宝贵的资源.
- 在发育过程中, lncRNA 进化趋向于专业化.
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