纳米生物工厂:使用量子点-细菌纳米生物混合物的光驱动可再生生物化学合成
Yuchen Ding1,2,3, John R Bertram3,4, Carrie Eckert3,5
1Chemical and Biological Engineering , University of Colorado Boulder , Boulder , Colorado 80303 , United States.
Journal of the American Chemical Society
|June 28, 2019
概括
使用光能将二氧化碳,水和转化为生物燃料和化学物质. 这些纳米生物混合生物作为高效的光驱纳米微生物工厂.
科学领域:
- 生物技术
- 纳米技术
- 合成生物学
背景情况:
- 活细胞自然不会与纳米级材料交互.
- 有纳米级组件的人造生物具有独特的多功能性质.
- 纳米级材料与细胞的接口需要化学,能量和生物相容的设计.
研究的目的:
- 通过与细菌合量子点 (QD) 来创建纳米生物混合生物 (nanorgs).
- 通过使用二氧化碳,水和来实现光驱生物燃料和化学品的生产.
- 研究QD细胞合和纳米器官功能机制.
主要方法:
- 使用了七个不同的核心外量子点 (QD),具有不同的激发能量.
- 采用富含的QD外方面,以结合细菌蛋白质.
- 结合的氨酸联体用于细胞吸收和存活.
- 照明QD来驱动光诱导的减少反应.
主要成果:
- 通过紫外线到近红外光谱成功将QD与细菌中的酶部位结合起来.
- 生物燃料 (IPA,BDO,MKs,H2) 和化学品 (FA,NH3,C2H4,PHB) 的光驱生产已经证明.
- 实现了大约10^6-10^8mol的每mol细胞的高周转率.
- 已证实细胞生存和作为静止的纳米微生物工厂的功能.
结论:
- 通过将QD与细菌结合起来,开发出功能性纳米器官,用于可持续的化学和生物燃料生产.
- 使用工程纳米材料建立了一种光激活生物催化新方法.
- 突出了纳米器官作为高效的可再生能源驱动的细胞工厂的潜力.
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