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可激活的NIR光/MRI双模探头,用于通过酶介导化反应和自组装进行体内成像
Runqi Yan1, Yuxuan Hu1, Fei Liu1
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering , Nanjing University , Nanjing 210023 , China.
Journal of the American Chemical Society
|June 28, 2019
概括
研究人员开发了一种用于近红外光和磁共振成像的新型双模探测器. 这种探测器能够实时,高灵敏度地可视化活细胞和瘤中的性酸酶 (ALP) 活性.
科学领域:
- 生物医学工程
- 分子成像
- 纳米技术
背景情况:
- 小分子自组成纳米结构的刺激反应显示出分子成像和组织工程的前景.
- 在单个小分子探测器内同时激活多模式成像信号仍然是一个重大挑战.
研究的目的:
- 设计和开发基于小分子的可激活近红外 (NIR) 光和磁共振 (MR) 双模探头.
- 将化反应与酶响应的现场自组合集成,以增强分子成像.
主要方法:
- 设计了一个双模探头 (P-CyFF-Gd),将化反应与酶响应自组合集成.
- 使用性酸酶 (ALP) 作为模型目标,利用其在细胞膜上的过度表达.
- 使用冷扫描电子显微镜 (cryo-SEM) 的可视化膜局部组装纳米粒子 (NP).
主要成果:
- 在ALP激活时同时达到70倍以上的NIR光增强和约2. 3倍的r1放松度增加.
- 在活瘤细胞和小鼠中实现实时,高灵敏度,高空间分辨率成像和ALP活动的定位.
- 成功地划定了正位肝脏瘤焦点,帮助手术内以图像为导向的手术切除.
结论:
- 开发的策略有效地将可激活的NIR光和MRI通过现场自组装用于ALP活动成像.
- 这种方法为设计其他可激活的双模探针提供了多功能平台,用于实时体内酶活性和位置成像.
- 这种探测器可以有效地进行图像导向的瘤切除,突出显示其潜在的临床应用.
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