光具有改进的光物理特性和细胞透性,由体复合形成提供
Prem N Basa1, Chelsea A Barr1, Kady M Oakley1
1Department of Chemistry and Biochemistry , Worcester Polytechnic Institute , 100 Institute Road , Worcester , Massachusetts 01609-2280 , United States.
Journal of the American Chemical Society
|July 2, 2019
概括
研究人员开发了一种新的光 (XDPAdeCage),用于控制离子 (Zn2+) 释放. 这种方法可以使用光线对细胞信号通路进行精确的询问,从而为生物过程提供了新的见解.
科学领域:
- 生物化学 生物化学
- 化学生物学 化学生物学
- 细胞信号传输 细胞信号传输
背景情况:
- 光复合体为生物研究提供了对金属离子可用性的时空控制.
- 了解细胞信号通路通常需要精确操纵细胞内金属离子度,特别是 (Zn2+).
研究的目的:
- 开发和描述一种新的光,XDPAdeCage,用于光诱导的Zn2+释放.
- 为了评估XDPAdeCage的光物理特性和Zn2+结合特性.
- 为了证明XDPAdeCage对研究细胞内Zn2+动态和下游细胞反应的实用性.
主要方法:
- 合成和特征的光 {bis[(2-pyridyl) 甲基]氨基} ((9-oxo-2-xanthenyl) 乙酸 (XDPAdeCage).
- 光解实验以确定量子产量和解动力学.
- 异热定位热量计用于测量Zn2+结合亲和力.
- 用于细胞内输送的膜透三元复合物的制备.
- 光成像和RT-PCR用于评估Zn2+运输和细胞反应.
主要成果:
- XDPAdeCage表现出高效的光解与27%的量子产量.
- 光以高亲和力 (4.6 pM) 结合了Zn2+ ,在光解时释放出来.
- 2 - 桑乙酸染色体在红移激发和灭绝系数方面具有优势.
- 中性三元复合体是膜通透的,促进细胞内Zn2+释放.
- 光成像证实了Zn2+运输,RT-PCR显示了基因表达的改变.
结论:
- XDPAdeCage是一种强大的光,用于控制Zn2+释放,使细胞信号的时空控制成为可能.
- 开发的光及其膜通透复合体为研究细胞内Zn2+动态提供了一个非侵入性的工具.
- 这种方法有助于研究Zn2+介导的细胞过程和基因表达变化.
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