对于Fos蛋白复合体和转录因子AP-1的常见DNA结合部位
F J Rauscher1, L C Sambucetti, T Curran
1Department of Molecular Oncology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Cell
|February 12, 1988
概括
蛋白复合体与脂肪细胞P2基因调节元件 (FSE2) 结合,结合水平与的表达直接相关. 这种结合位点也被转录因子AP-1识别,这表明了功能链接.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 脂肪细胞P2 (aP2) 基因对于脂肪细胞的分化至关重要.
- 在aP2基因中的一个调节元件FSE2结合了包括Fos原型瘤基因产物在内的蛋白质复合体.
- 在分化过程中调节FSE2结合过程中Fos的确切作用需要进一步阐明.
研究的目的:
- 研究Fos表达水平与FSE2结合复合体之间的关系.
- 识别FSE2.2中含有Fos的复合体识别的特定DNA序列.
- 探索Fos与转录因子AP-1之间的功能联系.
主要方法:
- 在具有不同Fos表达的细胞中分析FSE2结合复合体.
- 对DNA-蛋白质复合物的免疫阻塞分析.
- 对FSE2序列的突变分析.
- DNA结合竞争试验. 竞争试验.
- 使用对Fos和Jun.的抗体进行光交叉链接和免疫沉降.
主要成果:
- FSE2结合复杂水平在数量和质量上反映了Fos的表达.
- 的表达诱导显著增加了FSE2结合复合体.
- 福斯复合体识别了TGACTCA序列,这是AP-1和GCN4.4已知的结合部位.
- 与AP-1和Fos相关的蛋白质具有类似的DNA识别特性.
结论:
- 蛋白直接影响与FSE2调节元件的结合.
- 鉴定的TGACTCA结合部位表明Fos在AP-1-介导的转录中发挥了直接作用.
- 这些发现表明Fos与AP-1转录因子复合体之间的功能关系.
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