在Bacillus subtilis中处理一个分泌西格玛因子:形态结构如何控制基因表达
P Stragier1, C Bonamy, C Karmazyn-Campelli
1Institut de Microbiologie, Université Paris-Sud, Orsay, France.
Cell
|March 11, 1988
概括
细菌细菌的分泌过程涉及到由SpoIIGA蛋白调节的pro-sigma E处理. 这个过程将形态变化与发育过程中的基因表达时间联系起来.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 发育生物学 发展生物学
背景情况:
- 细菌细菌的分泌与基因表达时间的形态变化相结合.
- 西格玛E (σE) 是一个关键的发育性西格玛因子,合成为非活性前体 (pro-σE).
- 早期的转录控制依赖于pro-σE的激活.
研究的目的:
- 研究 spoIIGA 基因在 E 处理中所扮演的角色.
- 了解将形态发展与西格玛E激活联系在一起的机制.
- 为了确定SpoIIGA的pro-sigma E处理活动的触发因素.
主要方法:
- 在 Bacillus subtilis 中 spoIIGA 突变的遗传分析.
- 在植物细胞中研究sigE和spoIIGA的表达.
- 对SpoIIGA多结构和合成时间的分析.
主要成果:
- 在spoIIGA中发生的突变阻止了pro-sigma E处理.
- 在植物细胞中,spoIIGA和sigE的同时表达会激活SIGMA E受控的促进体.
- 在检测其处理活动之前,合成了一种膜蛋白SpoIIGA.
- 似乎SpoIIGA的处理活动是由分泌隔膜触发的.
结论:
- 斯波伊尔加拥有pro-sigma E处理业务.
- 根据spoIIAA和spoIIE,分泌隔膜可能会触发SpoIIGA的活动.
- 这种机制提供了形态结构和发育基因表达调节之间的直接联系.
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