相关实验视频

Updated: Jan 22, 2026

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

内源G蛋白结合受体的基因向光学控制

Prashant C Donthamsetti¹, Johannes Broichhagen², Vojtech Vyklicky¹

¹Department of Molecular and Cell Biology , University of California , Berkeley , California 94720 , United States.

Journal of the American Chemical Society

|July 11, 2019

概括

研究人员开发了一种新方法,maPORTL,使用光精确控制内源G蛋白合受体 (GPCR). 这一突破为研究生物系统中的GPCR功能提供了前所未有的时间和空间准确性.

科学领域:

神经科学
分子生物学
生物化学

背景情况:

G蛋白结合受体 (GPCR) 是关键的膜蛋白,但对它们在生物系统中的功能进行精确控制仍然具有挑战性.
像DREADDs和optoXRs这样的现有工具使用工程蛋白质,其表达,局部化和与内源性合作伙伴的相互作用可能受到限制.
目前基于连接体的方法缺乏时间控制,限制了它们在动态生物学研究中的有用性.

研究的目的:

开发一种用于精确控制内源性GPCR的新方法.
调查内源性转基因谷氨酸受体2 (mGluR2) 在主皮质神经元中的功能.
为研究具有高空间时间分辨率的内源GPCR信号建立一个多功能平台.

主要方法:

使用化学,生物学和光的组合来控制内源mGluR2,一个C家族的GPCR.
开发了一种膜定可光切换的直角远程连接联体 (maPORTL) 系统.
使用可光切换的谷氨酸与基因向的血点进行受体激活.

主要成果:

在初级皮层神经元中实现内源mGluR2的快速,可逆和选择性激活.
证明光激活可以通过修改PORTL长度和膜属性来调整.
成功控制了具有细胞类型特异性和高时空精度的内源GPCR信号.

结论:

maPORTL系统为精确控制内源GPCR提供了一个强大的新工具.
这种方法通过使用原生受体和光感应控制来克服现有方法的局限性.
这些发现为在各种生物环境中研究其他内源GPCR开发类似系统提供了一个模板.

更多相关视频

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

Quantifying Agonist Activity at G Protein-coupled Receptors

Quantifying Agonist Activity at G Protein-coupled Receptors

Published on: December 26, 2011

相关概念视频

G-protein Coupled Receptors

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.

G Protein-coupled Receptors

G Protein-coupled Receptors

G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...

Transducer Mechanism: G Protein–Coupled Receptors

Transducer Mechanism: G Protein–Coupled Receptors

G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...

Internal Receptors

Internal Receptors

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.

What is Genetic Engineering?

What is Genetic Engineering?

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis