相关实验视频
Updated: Jan 22, 2026
08:04
Conditions Affecting Social Space in Drosophila melanogaster
Published on: November 5, 2015
12.8K
在营业额条件下,异构的ABC出口商的整合空间
Susanne Hofmann1, Dovile Januliene2, Ahmad R Mehdipour3
1Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Frankfurt am Main, Germany.
Nature
|July 19, 2019
概括
这项研究揭示了不对称的ATP结合盒 (ABC) 导出器是如何通过捕获分子机器而起作用的. 酸盐释放,而不是ATP水解,驱动这些关键膜蛋白的结构变化.
科学领域:
- 结构生物学
- 生物化学
- 膜蛋白动力学
背景情况:
- ATP结合盒 (ABC) 出口物是细胞过程,免疫力和耐药性至关重要的动态膜蛋白.
- 了解非对称的ABC出口商在其功能周期中的构造变化至关重要,但仍未得到解决.
- 这些载体从细胞质中挤出各种物质,影响细胞功能和疾病状态.
研究的目的:
- 使用高分辨率冷电子显微镜 (cryo-EM) 划出一个不对称的ABC出口器的完整功能周期.
- 解决核酸结合,水解和释放到这些输送器的结构动态.
- 捕捉和描述基质转移周期的短暂中间状态.
主要方法:
- 使用了八种高分辨率的冷电子显微镜 (cryo-EM) 结构.
- 在活跃的营业环境下分析的ABC出口商.
- 使用突变和化学调制来捕获关键的动力中间体.
主要成果:
- 捕获了不同的向内 (IF) 形状,包括一个带有结合基质的形状.
- 识别了具有二元化核酸结合域和封闭的细胞外门的新型不对称的溶解后状态.
- 通过调节ATP水解速率来解决面向外的 (OF) 开放形状,揭示了它的短暂性质.
- 证明了与ATP结合的预水解状态和被瓦纳基捕获的状态是相等的,显示了共存的OF状态.
- 在水解后的状态下观察到不对称的ATP和ADP封闭,与渐进的核酸结合域分离和细胞内门解锁.
- 确立了酸盐释放,而不是ATP水解,触发返回IF形态.
结论:
- 酸盐释放是非对称的ABC输出者恢复向内状态的关键触发因素.
- 该研究通过绘制它们的形状地图,解决了非对称的ABC输送器基质转位周期的基本步骤.
- 结合动态调制, 提供了前所未有的洞察力,
相关概念视频
01:31ABC Transporters: Exporter
6.3K
ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
6.3K
01:20Conformity
47.9K
Conformity is the change in a person’s behavior to go along with the group, even if that person does not agree with the group.
47.9K
01:42Nuclear Export
4.9K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
4.9K
01:27ABC Transporters: Importer
3.4K
ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
3.4K
02:19Export of Mitochondrial and Chloroplast Genes
4.1K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
4.1K
02:31Nuclear Export of mRNA
5.3K
5.3K