通过DNA设计的一种交叉连接方法来稳定响应刺激的体晶体
Seungkyu Lee1, Cindy Y Zheng1, Katherine E Bujold1
1Department of Chemistry , Northwestern University , Evanston , Illinois 60208 , United States.
Journal of the American Chemical Society
|July 19, 2019
概括
DNA交叉连接试剂提高了DNA工程结合体晶体的稳定性. 这些修改后的晶体在各种化学环境和pH水平中保持结构完整性,扩大了它们的潜在应用.
科学领域:
- 材料科学
- 生物技术
- 纳米技术
背景情况:
- 它们具有独特的结构性质.
- 现有的稳定方法在恶劣环境中存在局限性.
研究的目的:
- 研究使用有机DNA交联试剂来稳定DNA工程的合晶体.
- 评估交叉连接对晶体稳定性,秩序和灵活性的影响.
主要方法:
- 使用双乙氨酸urea和8-methoxypsoralen进行DNA链间基因对的共价交联.
- 使用小角度X射线散射 (SAXS) 进行晶体稳定性的表征和环境耐受性的评估 (溶剂,pH).
主要成果:
- 交叉连接显著提高了化学和热稳定性,而不会影响远程秩序.
- 经过修改的晶体在乙醇到六和pH0和14的溶剂中显示稳定性.
- 交联DNA保留了灵活性,允许依赖于溶剂的可逆晶格参数变化.
结论:
- 有机交叉链接是稳定DNA工程合晶体的有效策略.
- 这种方法为无机稳定技术提供了多功能替代方案.
- 稳定晶体在多样化和极端介质中的应用是有前途的.
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