用于膜潜在成像的BODIPY光体
Journal of the American Chemical Society
|July 25, 2019
概括
研究人员通过添加硫酸芳香基来开发出新的水溶性BODIPY染料. 这些染料有助于在细胞和神经元中产生电压敏感的光体 (VF).
科学领域:
- 有机化学
- 生物物理化学
- 材料科学
背景情况:
- 由于其可调性特性和生物兼容性,BODIPY染料是有价值的光体.
- 提高BODIPY染料的水溶性对于生物应用至关重要,但也带来了合成方面的挑战.
- 现有的水溶解方法可能会干扰功能指标所需的电子特性.
研究的目的:
- 开发一种用于制造水溶性BODIPY染料的总体策略.
- 为细胞成像合成基于BODIPY的新型电压敏感光体 (VF).
- 评估这些新染料在生物系统中的性能.
主要方法:
- 合成了五种新的BODIPY,
- 功能化这些水溶性BODIPY以创建13个基于BODIPY的新型电压敏感光体 (VF).
- 在哺乳动物细胞和神经元中测试了新的VF染料的电压敏感性和亮度.
主要成果:
- 证明在中位位置的硫化芳香基是水溶性BODIPY的有效策略.
- 开发了13种新的基于BODIPY的电压敏感光体.
- 在使用最敏感的染料的哺乳动物细胞中达到高电压敏感度 (48% ΔF/F/100 mV).
- 展示了这些染料在神经元和心肌细胞中的动力学报告的能力.
结论:
- 加入硫酸芳香基的策略为水溶性BODIPY光体提供了一种多功能途径.
- 开发的BODIPY VF为研究细胞电生理学提供了有前途的工具,其亮度和灵敏度得到了提高.
- 这项工作扩大了设计具有可调节电子属性的功能光指示器的工具包.
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