虫tRNA衍生的小RNA是调节植物结节的信号分子
Bo Ren1, Xutong Wang1, Jingbo Duan1
1Department of Agronomy, Purdue University, West Lafayette, IN 47907, USA.
概括
由虫转移RNA (tRNA) 衍生的小RNA片段 (tRF) 作为控制豆类结节的信号. 操纵这些细菌的tRF可以增强植物微生物的相互作用,提高作物产量.
科学领域:
- 微生物学
- 植物科学
- 分子生物学
背景情况:
- 豆类共生需要细菌和宿主植物之间的分子信号.
- 菌感染和根结的发展是复杂的过程.
- 小RNA是众所周知的各种生物过程的调节者.
研究的目的:
- 研究细菌小RNA在豆类结节中的作用.
- 确定涉及宿主基因调节的特定根茎小RNA.
- 探索利用这些小RNA的潜力,
主要方法:
- 菌转移RNA (tRNA) 衍生的小RNA片段 (tRF) 的鉴定和表征.
- 使用短串目标模仿 (STTM),人工微RNA (amiRNA) 和CRISPR-Cas9基因编辑对tRF进行实验操作.
- 对宿主基因表达和结节表型 (根毛,结节形成) 的分析.
- 研究宿主ARGONAUTE 1在RNA干扰途径中的参与.
主要成果:
- 确定了三种根茎类tRF作为调节宿主结节的关键信号分子.
- 这些tRF通过劫持ARGONAUTE 1介导的RNA干扰途径来调节控制结节的宿主基因.
- 抑制tRF或过度表达其点抑制结节的形成,而相反的操作则促进结节的形成.
- 细菌的tRF被证明可以调解 prokaryote- eukaryote 相互作用.
结论:
- 菌tRFs代表一种新型的细菌信号分子,可以调节豆类结节.
- 这种细菌小RNA介导机制为植物微生物相互作用提供了新的视角.
- 针对这些tRF提供了增强豆类共生效率和作物生产率的潜在策略.
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