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一个限制奖励动机的Paranigral VTA Nociceptin电路
Kyle E Parker1, Christian E Pedersen2, Adrian M Gomez1
1Departments of Anesthesiology, Division of Basic Research, Anatomy and Neurobiology, and Washington University Pain Center, Washington University School of Medicine, St. Louis, MO, USA.
Cell
|July 27, 2019
概括
研究人员发现了一种新型的大脑电路, 涉及腹膜区域 (VTA) 中的受素 (Pnoc) 神经元, 调节寻求奖励. 这种Pnoc-VTA途径限制了自然奖励的动机,影响了奖励行为.
科学领域:
- 神经科学
- 行为神经科学
- 分子精神病学
背景情况:
- 诺西塞丁及其受体 (NOPR) 存在于与奖励行为相关的大脑区域.
- 诺西塞信号传递在动机行为中的确切作用和时间尚不清楚.
研究的目的:
- 在寻求奖励的行为中研究诺西胺电路的功能.
- 确定参与调节动机的特定神经元群体和途径.
主要方法:
- 产生预先受精蛋白 (Pnoc) -Cre小鼠线来准特定的神经元.
- 在操作过程中记录神经元活动的光纤测量.
- 用于神经元操纵的光遗传和化学遗传技术.
- 在VTA中条件删除诺西受体 (NOPR).
主要成果:
- 鉴定出表达Pnoc的神经元的一个独特亚群.
- 当寻求奖励的行为停止时, pnVTA神经元活动达到峰值.
- 废除或抑制PNVTAPnoc神经元,或删除VTA NOPR,增强对奖励的反应.
- 激活PNVTAPnoc神经元导致避免奖励和减少动机.
结论:
- pnVTAPnoc核和VTA NOPR信号对于调节奖励动机至关重要.
- 这项研究揭示了一种新的神经调节电路,
- 这些发现提供了对激励行为神经基础的洞察.
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