该c-Fos蛋白与c-Jun/AP-1相互作用,以刺激AP-1响应基因的转录
1Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla 92093.
Cell
|August 12, 1988
概括
蛋白通过与AP-1转录因子相互作用,起到转录转激活剂的作用. 这种相互作用对于刺激基因表达至关重要,突出的是AP-1
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 转录因子 转录因子
背景情况:
- 对c-fos基因产物Fos进行了研究,以确定其作为转录转激活器的作用.
- 细胞系被设计为表达可诱导的MT-fos仿真基因,用于研究Fos功能.
- 以前的研究表明,Fos参与了基因表达调节.
研究的目的:
- 确定Fos是否可以作为转录的转激活剂.
- 阐明Fos调解基因表达的机制.
- 研究AP-1转录因子在Fos介导激活中的作用.
主要方法:
- 细胞系 (3T3MTfos和F9MTfos) 的稳定转染与金属诱导的MT-fos基因.
- 记者基因测试测量AP-1响应基因转激活.
- 通过c-Jun/AP-1表达载体和共免疫沉试验传染.
主要成果:
- 在3T3MTfos细胞中,Fos表达诱导了AP-1记者基因的转激活.
- 斯诱导并没有激活缺乏AP-1的F9MT斯细胞中的记者基因.
- 在F9MTfos细胞中恢复c-Jun/AP-1表达,恢复了Fos介导的转激活.
- 发现在蛋白质复合体中与c-Jun/AP-1 (FAP p39) 相结合.
结论:
- 作为一种转基因作用因子,间接刺激基因表达.
- 的活性需要与特定序列的转录因子AP-1相互作用.
- 通过AP-1识别cis元素是Fos介导基因表达的先决条件.
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