相关的细胞p39与核转录因子AP-1有关
P Sassone-Corsi1, W W Lamph, M Kamps
1Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92138.
Cell
|August 12, 1988
概括
原瘤基因蛋白 (p55fos) 与蛋白 (p39jun) 形成复合体,以调节基因转录. 这种fos-jun复合体与TPA响应促进元件 (TREs) 结合,激活哺乳动物细胞中的转录.
科学领域:
- 分子生物学分子生物学
- 瘤发生的发生因子.
- 基因规则 基因规则
背景情况:
- 原始瘤基因fos编码了一种参与转录调节的核蛋白 (p55fos).
- p55fos与核蛋白结合,在结合TPA响应性促进元件 (TREs) 的复合体中.
- 核原基因结编码激活蛋白1 (AP-1),这是TREs的特定结合因子.
研究的目的:
- 为了研究关联蛋白和AP-1之间的关系.
- 为了确定fos和jun蛋白质是否形成调节转录的功能复合体.
主要方法:
- 对相关蛋白质的免疫学和结构分析.
- 使用p39.9.的凝净化和DNA结合试验.
- 在HeLa细胞提取物中分析核蛋白复合体.
- 在哺乳动物细胞中进行传染试验,以评估转录激活.
主要成果:
- 一种与fos相关的蛋白质,p39,与AP-1具有免疫学和结构上的相似性.
- p39与AP-1共识绑定站点 (TGACTCA) 相结合.
- p55fos和p39jun形成了一个核蛋白复合体,与HeLa细胞提取物中的TRE相互作用.
- 和之间的合作对于对TPA响应性促进体的完全转录激活至关重要.
结论:
- 核coproteins 和合作形成一个功能转录因子复合体.
- 这种fos-jun复合体与TRE结合,并激活来自TPA响应性促进体的转录.
- 这些发现阐明了涉及原型瘤基因的转录调节中的一个关键机制.
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