素采集是一种抗氧化策略,并触发地下聚胺代谢
Viridiana Olin-Sandoval1,2, Jason Shu Lim Yu3, Leonor Miller-Fleming1,4
1Department of Biochemistry, University of Cambridge, Cambridge, UK.
Nature
|August 2, 2019
概括
酵母细胞通过改变新陈代谢来适应氧化应激. 它们吸收氨酸,从而促进聚胺的产生,增加谷氨和增强氧化剂耐受性.
科学领域:
- 细胞生物学
- 生物化学
- 微生物学
背景情况:
- 有机体依赖于对环境变化的抗压机制.
- 代谢变化,比如糖溶解到酸盐路径,是关键的应激反应.
- 酵母聚胺载体Tpo1p在氧化剂耐受性的作用尚不清楚.
研究的目的:
- 研究微生物应激抵抗的代谢适应.
- 在压力下探索聚胺和氨酸代谢之间的联系.
- 了解营养摄入如何影响压力保护.
主要方法:
- 蛋白质的时间过程实验以确定代谢链接.
- 涉及聚胺通路酶的酶反应的分析.
- 测量营养吸收,氧化还原代谢和活性氧物种水平.
主要成果:
- 酵母 Spe1p 酶不间断地去碳化 lysine 形成 cadaverine.
- 在压力下,细胞外素吸收显著增加,这取决于多胺通路.
- 这触发了代谢重编程,将NADPH引导到谷代谢中.
- 增加的谷氨酸水平导致反应性氧物种减少和氧化剂耐受性增加.
结论:
- 营养摄入不仅有助于生长,而且有助于在有利条件下进行新陈代谢.
- 聚胺和氨酸代谢之间的新联系提供了预防性应激保护机制.
- 这种代谢适应增强了微生物对抗氧化攻击的生存能力.
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