短α,β,γ混合的折叠和组装:序列的微小变化和更高层结构的剧烈差异
Yukun Zhang1,2, Yulong Zhong3, Alan L Connor3
1Chengdu Institute of Organic Chemistry , Chinese Academy of Sciences , Chengdu 610041 , China.
Journal of the American Chemical Society
|August 6, 2019
概括
七个短意外地折叠成新的结构, 包括扩展的β转和双螺旋, 而不是预期的配对. 这揭示了合成折叠剂的独特折叠原型.
科学领域:
- 超分子化学
- 的化学成分
- 结构生物学
背景情况:
- 蛋白质折叠通常需要长的多.
- 短通常缺乏明确的结构.
- 包含α和β氨基酸的混合提供了新的结构可能性.
研究的目的:
- 研究短混合的折叠和组装.
- 探索中心二序列对折叠的影响.
- 确定合成折叠材料的新型折叠原型.
主要方法:
- 核磁共振 (NMR) 光谱学
- 质谱学
- 计算研究
- 系统推断
主要成果:
- 聚合物1形成了混合氨基酸序列的扩展β转变.
- 2a和2b采用了扩展的螺旋形状,并二重化成双螺旋.
- 中心二序列 (αβ与 βα) 决定了明显的分子内结和折叠.
- 衍生品表现出一般的折叠行为,具有增强的稳定性和明确的螺旋感.
结论:
- 短混合可以形成独特的非正规结构,如扩展的β转和双螺旋.
- 合成折叠机提供了具有可调节性质的新型折叠原型.
- 这项工作扩大了对折叠和自我组装的理解.
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