合成序列纠增加了细胞中遗传信息的稳定性和封闭性
Tomasz Blazejewski1,2, Hsing-I Ho1, Harris H Wang3,4
1Department of Systems Biology, Columbia University, New York, NY, USA.
概括
科学家开发了一种使用重叠基因的计算策略,以稳定遗传功能并防止合成生物学中的DNA转移. 这种方法提高了工程生物的遗传稳定性和生物封闭性.
科学领域:
- 合成生物学
- 遗传学
- 分子生物学
背景情况:
- 基因工程需要确保稳定性和防止DNA逃逸的方法.
- 自然突变和基因流动对工程遗传功能构成挑战.
研究的目的:
- 通过重叠的基因来保存和限制遗传信息.
- 在合成生物学应用中增强遗传稳定性和生物控制.
主要方法:
- 基因重叠的计算设计.
- 通过重叠序列改变基本基因的适应性.
- 在有趣的基因中嵌入毒素基因.
- 对超过7500个重叠的序列设计进行多重和可扩展的测试.
主要成果:
- 叠加的序列限制了进化路径,即使是同义突变.
- 嵌入的毒素基因限制了水平基因的传播.
- 从自然同类产生的功能性,高度分离的变体.
结论:
- 使用重叠基因的策略是有效的基因稳定和生物控制.
- 这种方法有助于更深入地探索自然和人工重叠的基因.
- 在新兴合成生物学应用中增强遗传稳定性和生物控制.
相关概念视频
RNA Stability
35.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.6K
Nuclear Stability
22.9K
Protons and neutrons, collectively called nucleons, are packed together tightly in a nucleus. With a radius of about 10−15 meters, a nucleus is quite small compared to the radius of the entire atom, which is about 10−10 meters. Nuclei are extremely dense compared to bulk matter, averaging 1.8 × 1014 grams per cubic centimeter. If the earth’s density were equal to the average nuclear density, the earth’s radius would be only about 200 meters.
To hold positively charged protons together...
To hold positively charged protons together...
22.9K
What is Genetic Engineering?
79.7K
Overview
79.7K
Synthetic Biology
5.5K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Golden rice
Golden rice is a genetically modified...
5.5K
Animal Mitochondrial Genetics
9.0K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.0K
Stability
392
The time response of a linear time-invariant (LTI) system can be divided into transient and steady-state responses. The transient response represents the system's initial reaction to a change in input and diminishes to zero over time. In contrast, the steady-state response is the behavior that persists after the transient effects have faded.
The stability of an LTI system is determined by the roots of its characteristic equation, known as poles. A system is stable if it produces a bounded...
The stability of an LTI system is determined by the roots of its characteristic equation, known as poles. A system is stable if it produces a bounded...
392


