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Gene Expression Analyses in Human Follicles
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人类微生物组的大规模分析揭示了成千上万个小的新基因
Hila Sberro1, Brayon J Fremin2, Soumaya Zlitni2
1Department of Medicine (Hematology; Blood and Marrow Transplantation) and Genetics, Stanford University, Stanford, CA, USA; Department of Genetics, Stanford University, Stanford, CA, USA.
Cell
|August 13, 2019
概括
研究人员在人体微生物组中发现了4000多个保存的小蛋白家族,其中许多具有新性和潜在的新功能. 这一发现凸显了小蛋白在人类健康和微生物群落中所扮演的角色.
科学领域:
- 基因组学
- 微生物学
- 蛋白质组学
背景情况:
- 很难检测到小蛋白质,导致它们在生物研究中的代表性不足.
- 了解小蛋白的功能作用对于理解生物系统至关重要.
研究的目的:
- 系统地识别和描述人类微生物组中保存的小蛋白家族.
- 研究这些新发现的小蛋白质的潜在功能和起源.
主要方法:
- 对来自不同身体部位的1773个人类相关基因组进行比较基因组学分析.
- 蛋白质局部化的生物信息预测 (分泌/跨膜) 和域识别.
- 蛋白家族起源的分析,包括潜在的水平基因转移和哺乳动物特异性.
主要成果:
- 鉴定了4000多个保存的小蛋白家族,其中大多数是新鲜的.
- 预计这些家族中的大约30%是分泌或跨膜的.
- 超过90%的小蛋白家族缺乏已知域,几乎一半的基因组缺失.
- 发现了可谓的家庭管理,哺乳动物特异性,与防御相关的和水平转移的蛋白质家族.
- 一些已识别的小蛋白质的转录和翻译证据.
结论:
- 人类微生物组中大量存在小蛋白质, 它们的功能还在很大程度上未被探索.
- 这些新型的小蛋白质可能在宿主-微生物相互作用和人类生物学中发挥着多种,以前未报告的作用.
- 这项研究为进一步实验性研究小蛋白的功能提供了基础.
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