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Induced-fit Model01:13

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Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
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Goodness-of-Fit Test01:16

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The goodness-of-fit test is a type of hypothesis test which determines whether the data "fits" a particular distribution. For example, one may suspect that some anonymous data may fit a binomial distribution. A chi-square test (meaning the distribution for the hypothesis test is chi-square) can be used to determine if there is a fit. The null and alternative hypotheses may be written in sentences or stated as equations or inequalities. The test statistic for a goodness-of-fit test is given as...
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Most altruistic behavior—in which one animal helps another at a cost to themselves—occurs between relatives. Scientists think these altruistic behaviors evolved because they increase the inclusive fitness of the animal providing help.
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Atoms and molecules interact through bonds (or forces): intramolecular and intermolecular. The forces are electrostatic as they arise from interactions (attractive or repulsive) between charged species (permanent, partial, or temporary charges) and exist with varying strengths between ions, polar, nonpolar, and neutral molecules. The different types of intermolecular forces are ion–dipole, dipole–dipole, hydrogen bonds, and dispersion; among these, dipole–dipole, hydrogen...
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Electricity is generated by either electrons or ions flowing through a solution or a conducting medium. This flow of electrons or specifically electrical charge is defined as an electric current. When electrons move through a wire, they generate an electric current. It can be recalled  that in a redox reaction, electrons are lost and gained. In the spontaneous redox reaction of zinc  with copper, when zinc is immersed in a copper ion solution, a transfer of electrons from one substance to...
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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
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强制插入 (FIT) - 适应剂

Sasha B Ebrahimi, Devleena Samanta, Ho Fung Cheng

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    此摘要是机器生成的。

    研究人员开发了一种新的信号吸收器类别,即强制插入吸收器 (FIT). 这些体采用单一的修饰,使其能够在复杂的生物样本中检测出各种光的敏感性和特异性.

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    科学领域:

    • 生物化学
    • 分子生物学
    • 分析化学

    背景情况:

    • 胺是用于分析物检测的多功能寡核酸工具.
    • 现有的aptamer信号方法往往有错误的阳性或有限的动态范围.

    研究的目的:

    • 为强大的光读取制定一个一般的aptamer设计策略.
    • 创建一个具有改进性能特性的新类信号体.

    主要方法:

    • 使用一种粘度敏感的染料,
    • 开发了强制合 (FIT) 合体,在合目标时增加光.
    • 在各种结合方式中展示了FIT-aptamer的实用性,包括构造变化,三明治测试和分裂-aptamer重组.

    主要成果:

    • 在目标结合时,FIT- 吸收剂的光度增加了20倍.
    • 该策略成功检测了金属基对的形成.
    • 与传统系统相比,FIT吸收器表现出较少的假阳性信号.
    • 在人血清中达到高信号-背景比率 (高达FRET的15倍) 和纳米分子灵敏度.

    结论:

    • FIT-aptamers代表了一种单一修改的新型信号aptamers.
    • 这种策略可以在复杂的媒介中对各种目标进行敏感和具体的检测.
    • 基于FIT的探测方法提供了一个有前途的替代方法.