单原子光开关:用于生物成像的可见光激活染料的一般方法
Juan Tang, Michael A Robichaux1, Kuan-Lin Wu
1Verna and Marrs McLean Department of Biochemistry and Molecular Biology , Baylor College of Medicine , Houston , Texas 77030 , United States.
Journal of the American Chemical Society
|August 28, 2019
概括
研究人员通过在常见染料中用硫代替氧气开发了新的可光激活光体. 这些化物能够有效地进行超高分辨率显微镜的光激活,在生理条件下提供卓越的性能.
科学领域:
- 化学生物学
- 显微镜
- 分子成像
背景情况:
- 可光激活的光体对于高分辨率的细胞结构和动态成像至关重要.
- 现有的可光激活探头通常需要恶劣的条件或细胞毒性添加剂来激活.
研究的目的:
- 开发一种用于制造新型可光激活化染料的通用和简单的策略.
- 在超高分辨率显微镜技术中展示这些新染料的实用性.
主要方法:
- 在常见的光体结构 (thio-caging) 中通过替换单个硫原子来合成新型光体.
- 通过理论计算研究光火机制.
- 在可见光和空气下通过脱硫证明光激活.
- 在光激活定位显微镜 (PALM) 中应用开发的探针.
主要成果:
- 已经成功合成了一系列可光激活的化染料.
- 通过光诱导的电子转移有效灭光.
- 在暴露在可见光和空气中时,获得了有效的光激活和光恢复.
- 在低激活光强度和没有细胞毒性添加剂的生理条件下使用PALM实现高分辨率成像.
- 证明了细胞组件的多色超高分辨率成像.
结论:
- 化策略为产生可光激活光体提供了一种多功能且有效的方法.
- 这些新型探测器为超分辨率成像提供了显著的优势,特别是在生物相关的条件下.
- 开发的光体是先进的细胞成像应用的有希望的工具.
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