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通过 Ms4a3-表达史痕迹的命运映射单细胞衍生细胞

Zhaoyuan Liu¹, Yaqi Gu¹, Svetoslav Chakarov²

¹Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

|September 7, 2019

概括

这项研究引入了Ms4a3作为追踪单细胞的新标志物. 研究人员精确量化了在恒常和炎症期间单细胞对组织居民巨细胞群的贡献.

科学领域:

免疫学
血液形成
细胞生物学

背景情况:

组织寄存巨细胞 (RTM) 主要是在胚胎发育和自我维持过程中形成的.
一个RTM子集由循环单细胞不断补充,但它们的贡献和功能仍然不太清楚.
在各种生理和病理状态下,单细胞衍生的RTM的确切程度和动力学受到争议.

研究的目的:

开发一种可靠的方法来追踪RTM群体中的单细胞衍生细胞.
在平衡和炎症期间量化循环单细胞对RTM的贡献.
建立单细胞衍生RTM的未来功能研究的基础.

主要方法:

确定Ms4a3作为颗粒细胞-单细胞原体 (GMP) 的特定基因标记物.
产生 Ms4a3 报告者 (Ms4a3^TdT),Cre (Ms4a3^Cre) 和可诱导的 Cre (Ms4a3^CreERT2) 鼠标模型.
使用命运映射策略追踪单细胞和颗粒细胞群,不包括淋巴细胞和组织树突细胞.

主要成果:

在GMP中证实了Ms4a3的表达,使得特定的血统可以追踪.
开发的Ms4a3命运映射模型准确地追踪了单细胞和颗粒细胞.
在平衡和炎症期间,可以精确量化单细胞对RTM池的贡献.

结论:

Ms4a3 作为特定和有效的标记物用于追踪单细胞和颗粒细胞系.
这项研究为RTM群体中单细胞衍生细胞的明确识别提供了强有力的工具.
已建立的模型将促进对单细胞衍生的RTM在健康和疾病中的功能作用的未来研究.

关键词:

良好的营养美国没有.树突细胞运气测绘粒细胞-单细胞原始体发生炎症单细胞单细胞状细胞的原始细胞组织寄存的巨细胞

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