相关实验视频

Updated: Jan 19, 2026

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
09:33

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Published on: October 3, 2019

11.5K

迁移神经细胞 发育中的神经系统中的死细胞

Yunlu Zhu1, Samantha C Crowley1, Andrew J Latimer1

  • 1Department of Biology, University of Virginia, Charlottesville, VA 22904, USA.

Cell
|September 10, 2019
PubMed
概括

神经细胞在神经系统发育过程中清除细胞碎片,然后才有专业的细胞. 这些NCC表现出类似巨细胞的细胞,这对早期神经生成至关重要.

关键词:
介质蛋白-1β巨细胞神经细胞神经发育神经发生细胞发生斑马鱼

更多相关视频

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures09:26

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

Published on: June 27, 2022

3.0K
Isolating Cranial Neural Folds From a Chick Embryo for a Neural Crest Cell Culture03:34

Isolating Cranial Neural Folds From a Chick Embryo for a Neural Crest Cell Culture

539

相关概念视频

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration09:33

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

This protocol describes the dissection and culture of cranial neural crest cells from mouse models, primarily for the study of cell migration. We describe the live imaging techniques used and the analysis of speed and cell shape...
11.5K
Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures09:26

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

This versatile protocol describes the isolation of premigratory neural crest cells (NCCs) through the excision of cranial neural folds from chick embryos. Upon plating and incubation, migratory NCCs emerge from neural fold explants, allowing for assessment of cell morphology and migration in a simplified 2D environment.
3.0K
Isolating Cranial Neural Folds From a Chick Embryo for a Neural Crest Cell Culture03:34

Isolating Cranial Neural Folds From a Chick Embryo for a Neural Crest Cell Culture

This video illustrates a technique for isolating cranial neural folds from the midbrain region of chick embryos. It presents a detailed dissection process of the neural folds, their placement, and attachment on fibronectin-coated coverslips, followed by the observation of neural crest cell migration from the neural...
539
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells10:33

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Neural crest (NC) cells derived from human pluripotent stem cells (hPSC) have great potential for modeling human development and disease and for cell replacement therapies. Here, a feeder-free adaptation of the currently widely used in vitro differentiation protocol for the derivation of NC cells from hPSCs is...
14.6K
Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures08:29

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

Here we show our method to isolate mouse epidermal neural crest stem cells (EPI-NCSC). Technique involves micro-dissecting whisker follicles, isolating the bulge and placeing it into tissue culture. EPI-NCSC start to emigrate from bulge explants onto the substratum within 3 - 4...
11.9K
Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay12:17

Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay

An approach to analyze the migration of explanted cells (trunk neural crest cells) is described. This method is inexpensive, gentle, and capable of distinguishing chemotaxis from both chemokinesis and other influences on migratory polarity such as those derived from cell-cell interactions within the primary trunk neural crest cell...
12.8K