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Updated: Jun 15, 2026

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers
10:08

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

使用时间解析的3D单分子追踪方法测量活细胞中的DNA混合动力

Yuan-I Chen1, Yin-Jui Chang2, Trung Duc Nguyen1

  • 1Department of Biomedical Engineering , University of Texas at Austin , Austin , Texas 78712 , United States.

Journal of the American Chemical Society
|September 12, 2019
PubMed
概括

研究人员开发了一种3D追踪方法来观察活细胞中的DNA和融化. 与体外条件相比,细胞环境中的DNA杂交速度显著提升.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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