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相关概念视频

Evidence-based Knowledge Synthesis and Hypothesis Validation: Navigating Biomedical Knowledge Bases via Explainable AI and Agentic Systems05:47

Evidence-based Knowledge Synthesis and Hypothesis Validation: Navigating Biomedical Knowledge Bases via Explainable AI and Agentic Systems

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This article describes RUGGED (Retrieval Under Graph-Guided Explainable disease Distinction), which integrates Large Language Model (LLM) inference with Retrieval-Augmented Generation (RAG). It draws evidence from expert-curated biomedical knowledge bases and peer-reviewed biomedical publications to synthesize new knowledge from up-to-date information, identify explainable and actionable predictions, and pinpoint promising directions for hypothesis-driven...
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A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles08:31

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

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Here, we present a protocol for fabrication of a closed-type wireless nanopore electrode and subsequent electrochemical measurement of single nanoparticle...
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Chromatography-based Biomolecule Purification Methods07:51

Chromatography-based Biomolecule Purification Methods

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In biochemistry, chromatography-based purification methods are employed to isolate compounds from a complex mixture. Two such methods used commonly by biochemists are size-exclusion chromatography and affinity chromatography. In size-exclusion chromatography, a column packed with porous beads separates components of a mixture based on size. On the other hand, affinity chromatography allows for a more specific separation of biomolecules by using a column that is composed of stationary phase,...
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Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

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The presented protocol describes the analysis of membrane protein mediated transport on the single transporter level using pore-spanning solvent-free lipid bilayers. This is achieved by the creation of bulk produced nanopore array chips, combined with highly parallel data acquisition and analysis, enabling the future establishment of membrane protein effector...
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Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

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A methodology for preparing solid-state nanopores in solution for biomolecular translocation experiments is presented. By applying short pulses of high electric fields, the nanopore diameter can be controllably enlarged with subnanometer precision and its electrical noise characteristics significantly improved. This procedure is performed in situ using standard laboratory equipment under experimental...
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Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example05:45

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We previously validated a protocol for amplicon-based whole genome Usutu virus (USUV) sequencing on a nanopore sequencing platform. Here, we describe the methods used in more detail and determine the error rate of the nanopore R10 flow...
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相关实验视频

Updated: Jan 19, 2026

Evidence-based Knowledge Synthesis and Hypothesis Validation: Navigating Biomedical Knowledge Bases via Explainable AI and Agentic Systems
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基于纳米孔的单个生物分子接口:从信息到知识

Yi-Lun Ying1,2, Yi-Tao Long1,2

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering , Nanjing University , Nanjing 210023 , P. R. China.

Journal of the American Chemical Society
|September 12, 2019
PubMed
概括

生物纳米孔作为单分子传感器来捕获和识别分子. 未来的研究旨在将它们的使用范围扩大到DNA测序之外,用于各种生物检测.

科学领域:

  • 生物物理
  • 分子生物学
  • 纳米技术

背景情况:

  • 单分子测量对于理解生物系统至关重要.
  • 生物纳米孔是一种膜蛋白,为单个分子提供了有限的空间.
  • 这些纳米孔作为捕获和识别单个生物分子的接口, 作为传感器.

研究的目的:

  • 概述基于生物纳米孔的单生物分子接口的设计.
  • 突出单个生物分子检测的未来研究方向.
  • 讨论纳米孔技术在新生物学问题上的潜力.

主要方法:

  • 专注于基于纳米孔的接口的设计原则.
  • 审查生物纳米孔的现有和潜在应用.
  • 讨论"单分子离子谱"的概念.

主要成果:

  • 纳米孔接口为每个生物分子提供丰富的随机信息.
  • 未来的应用包括罕见物种检测,中间识别和相互作用分析.
  • "单分子离子光谱"概念为原子级相互作用绘制提供了潜力.

结论:

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  • 生物纳米孔接口是单个生物分子检测的强大工具.
  • 在DNA测序之外扩展应用具有显著的前景.
  • 需要进一步的研究来应对挑战并释放新的生物学见解.