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相关概念视频

Controlled Strain of 3D Hydrogels under Live Microscopy Imaging07:41

Controlled Strain of 3D Hydrogels under Live Microscopy Imaging

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The presented method involves uniaxial stretching of 3D soft hydrogels embedded in silicone rubber while allowing live confocal microscopy. Characterization of the external and internal hydrogel strains as well as fiber alignment are demonstrated. The device and protocol developed can assess the response of cells to various strain...
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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana05:41

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

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This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing...
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Live Cell Imaging of Mitosis09:57

Live Cell Imaging of Mitosis

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Mitosis is a form of cell division in which a cell’s genetic material is divided equally between two daughter cells. Mitosis can be broken down into six phases, during each of which the cell’s components, such as its chromosomes, show visually distinct characteristics. Advances in fluorescence live cell imaging have allowed scientists to study this process in great detail, providing important insights into the biological control of this process and how it might go wrong in diseases...
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Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition10:21

Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition

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The current protocol describes a method by which users can maintain viability of acute hippocampal and cortical slice preparations during the collection of magnetic resonance microscopy...
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Long-term Live Imaging of Drosophila Eye Disc06:08

Long-term Live Imaging of Drosophila Eye Disc

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This protocol describes a detailed method for the long-term ex vivo culture and live imaging of a Drosophila imaginal disc. It demonstrates photoreceptor differentiation and ommatidial rotation within the 10 h period of live imaging of the eye disc. The protocol is simple and does not require expensive...
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Controlled Strain of 3D Hydrogels under Live Microscopy Imaging
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高尚的生活

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