用于从生物材料中释放模式蛋白质的基因编码的可光切割连接器

Biomaterials are materials engineered to interact favorably with biological organisms or molecules. These materials can be derived from or produced by an organism, or can even be a synthesized polymer. Engineers use these novel materials in a wide range of applications, such as tissue engineering, biosensing and drug delivery.
This video introduces common biologically derived materials, and provides examples of common techniques used to process them. Key challenges in the field are discussed,...

Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...

Here, a procedure to selectively activate a neuronal protein with a short pulse of light by genetically encoding a photo-reactive unnatural amino acid into a target neuronal protein expressed in neurons in culture or in vivo is...

We genetically-encode the unnatural amino acid, p-azido-L-phenylalanine at various targeted positions in GPCRs and show the versatility of the azido group in different applications. These include a targeted photocrosslinking technology to identify residues in the ligand-binding pocket of a GPCR, and site-specific bioorthogonal modification of GPCRs with a peptide-epitope tag or fluorescent...

This article will demonstrate how to monitor glutamine dynamics in live cells using FRET. Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed for genetically encoded glutamine...

Silk fibers have been processed and used to create fabrics and threads for centuries. However, the solubilizing of silk fibers, thereby turning it into a versatile pre-polymer solution is a much newer technology. Solubilized silk can be processed in many different ways to create a biocompatible material with controllable mechanical properties.
This video introduces the processing of silk from silk worm cocoons, and shows how the silk solution can be used to create a fiber mat via...