古代染色体的物理和功能细分
Naomichi Takemata1, Rachel Y Samson2, Stephen D Bell1
1Molecular and Cellular Biochemistry Department, Indiana University, Bloomington, IN, USA; Biology Department, Indiana University, Bloomington, IN, USA.
Cell
|September 21, 2019
概括
古代染色体具有不同的影响基因表达的领域,挑战了先前的模型. 一种新型的蛋白质 - - 凝聚素,将染色体组织与Sulfolobus中的基因活动联系起来.
科学领域:
- 微生物学
- 分子生物学
- 基因组学
背景情况:
- 染色体的三维组织影响复制和基因表达.
- 细胞染色体有不同的区块;细菌染色体有更简单的域受基因表达的影响.
- 染色体的结构维护 (SMC) 复合体,如凝聚素,形成更高阶的染色体结构.
研究的目的:
- 研究古生物,特别是Sulfolobus物种的高阶染色体组织.
- 确定古生物染色体是否具有与真核生物相似的分离区.
- 确定负责古染色体组织的分子因素及其与基因表达的联系.
主要方法:
- 在Sulfolobus物种上进行了染色体形状捕获 (3C) 实验.
- 与染色体域相关的基因表达模式的分析.
- 新型SMC超级家族蛋白的鉴定和表征.
主要成果:
- 硫球染色体表现出不同的域与特定的更高阶相互作用,形成两种分区类型.
- 分区身份与基因表达水平有因果关系.
- 一种以前未被描述的SMC超级蛋白,称为素,与这些域结合.
结论:
- 古代生物具有比以前假设的更复杂的染色体组织,具有明显的分离.
- 在定义染色体结构和调节 Sulfolobus 中基因表达方面,可莱辛起着至关重要的作用.
- 这一发现扩大了我们对不同生命领域的染色体组织的理解,特别是在没有正规凝聚素的情况下.
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