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基因组编辑追溯了君主抗毒素的演变

Marianthi Karageorgi¹, Simon C Groen^1,2, Fidan Sumbul³

¹Department of Integrative Biology, University of California, Berkeley, Berkeley, CA, USA.

|October 4, 2019

概括

蝶通过中的遗传变化 (Na+/K+-ATPase) 进化了对有毒植物的抵抗力. 这项研究使用基因编辑来创造

科学领域:

进化生物学
遗传学
生物化学

背景情况:

融合进化确定了适应性特征的基础突变.
植物中的心脏糖化物毒素对昆虫食草动物构成挑战.
(Na+/K+-ATPase) 是这些毒素的关键目标.

研究的目的:

研究昆虫适应心脏糖化物毒素的遗传基础.
阐明君主的进化抵抗路径.
验证特定突变在赋予耐药性的功能作用.

主要方法:

比较基因组学以确定ATPα中的平行氨基酸替代.
在Drosophila melanogaster中进行CRISPR-Cas9基编辑以模仿进化突变.
在体内,体外和体内分析以评估功能变化.

主要成果:

在适应过程中多次改变的ATPα中确定了三个关键氨基酸位点 (111,119,122).
具有三重突变的工程"君主"对心脏糖化物表现出抵抗力和不敏感性.
证明突变的顺序受到表皮症的影响,减轻了对抗性变性.

结论:

这项研究揭示了君主对心脏糖化物耐药性的特定遗传途径.
这种适应性使得君主能够隔离毒素, 威捕食者, 改变生态相互作用.
突出结合进化观察与基因组编辑的功能验证.

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