昆虫-细菌马赛克产生的糖
DeAnna C Bublitz1, Grayson L Chadwick2, John S Magyar2
1Division of Biological Sciences, University of Montana, Missoula, MT 59812, USA.
Cell
|October 8, 2019
概括
虫利用水平转移的细菌基因为内生生物莫拉内拉合成糖素 (PG). 这表明了昆虫和细菌基因组之间分布的功能途径,反映了器官遗传学.
科学领域:
- 微生物学
- 基因组学
- 进化生物学
背景情况:
- 糖素 (PG) 对细菌细胞结构和完整性至关重要.
- 虫拥有一个独特的结巢的内共生细菌系统 (特伦布莱亚内的摩兰氏菌).
- 之前的工作确定了PG生物合成基因的水平基因转移 (HGT) 从细菌到虫核基因组.
研究的目的:
- 研究横向转移的基因在虫内共生体中的功能整合.
- 阐明Moranella内生体中糖层形成的机制.
- 探索内生生物遗传与器官进化的相似之处.
主要方法:
- 虫和内生生物基因组的比较基因组学分析.
- 基因表达分析以确认功能途径.
- 用于追踪水平转移的昆虫蛋白质的蛋白质定位研究.
主要成果:
- 在虫基因组中水平转移的基因与Moranella保留的基因合作,在Moranella细胞外围形成PG.
- 一种来自横向转移的细菌基因的昆虫蛋白质存在于摩拉内拉细胞质中.
- 这建立了一个功能性的PG生物合成途径,分布在宿主和内生体基因组中.
结论:
- 这项研究表明,糖合成的功能途径分布在虫宿主及其内共生菌Moranella之间.
- 这种遗传和生化马赛克与有机细胞遗传系统具有惊人的平行性.
- 水平基因转移和整合可以导致对共生至关重要的复杂的多基因组功能途径.
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