20纳米以下的核心-外-外纳米颗粒用于亮度上升转换和增强的Förster共振能量传输
Chris Siefe1, Randy D Mehlenbacher1, Chunte Sam Peng2,3
1Department of Materials Science and Engineering , Stanford University , Stanford , California 94305 , United States.
Journal of the American Chemical Society
|October 9, 2019
概括
我们开发了一种新的核心-外-外纳米粒子设计, 显著提高生物成像和福斯特共振能量转移 (FRET) 应用的亮度. 这种小纳米粒子架构提高了信号噪声比和光学探测器效率.
科学领域:
- 纳米技术和材料科学
- 生物医学光学和成像
- 量子化学和光谱学
背景情况:
- 升级纳米粒子 (UCNPs) 是生物成像和福斯特共振能量转移 (FRET) 的有价值的光学探头,因为它们的信号与噪声比高,光稳定性和生物兼容性.
- 由于表面火,小型UCNP (<20 nm) 的亮度下降,限制了它们的应用.
- 现有增强UCNP亮度的方法通常会增加纳米粒子大小,从而抵消小型探针的需求.
研究的目的:
- 为小型,明亮的UCNP开发一种新的核心--纳米粒子架构.
- 优化UCNP设计以提高生物成像和FRET效率.
- 研究发射器度对纳米粒子性能的影响.
主要方法:
- 一个独特的核心--UCNP架构的制造:β-NaYbF4 (核心) @NaY0.8−xErxGd0.2F4 (内部) @NaY0.8Gd0.2F4 (外部).
- 在内部外中的发射器 (Er3+) 度的系统变化 (x = 180%).
- 与标准核心外UCNP架构 (β-NaY0.58Gd0.2Yb0.2Er0.02F4/NaY0.8Gd0.2F4) 的亮度,量子产量,寿命和FRET合的比较.
主要成果:
- 与标准核心外设计相比,核心外UCNP的单颗粒亮度高出2倍.
- 表面合FRET测试显示,使用核心-外-外架构提高了排放的8倍.
- 该研究确定了在新架构中最大限度地提高亮度和FRET效率的最佳发射度.
结论:
- 拟议的核心--架构允许创建适合先进生物成像和FRET应用的小型,明亮的UCNP.
- 这种设计通过优化离子分布和能量传输途径来克服小型UCNP的亮度限制.
- 对于设计具有卓越亮度和FRET能力的UCNP,仔细考虑发射度至关重要.
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