一种传感器酶控制着气驱动的菌感染植物
Lauren S Ryder1,2, Yasin F Dagdas1,2,3, Michael J Kershaw1
1Department of Biosciences, University of Exeter, Exeter, UK.
Nature
|October 11, 2019
概括
爆发性真菌使用传感器蛋白质Sln1来控制植物感染的内部压力. 这可以防止过度压力,并确保细胞正确组织以成功透宿主.
科学领域:
- 植物病理学
- 分子生物学
- 细胞生物学
背景情况:
- 爆发性真菌Magnaporthe oryzae通过appressoria感染植物,这些专门的细胞产生高气压力以破裂植物皮质.
- 调节压力形成的分子机制尚不清楚.
研究的目的:
- 调查西酸激酶Sln1在Magnaporthe oryzae中介的植物感染期间调节压的作用.
主要方法:
- 使用了Magnaporthe oryzae的一种Δsln1突变.
- 在压力下观察到Sln1的局部化.
- 分析了隔膜和极性决定者的组织.
- 研究了涉及Sln1,蛋白激酶C (Pkc1) 和蛋白激酶A (Pka) 的信号通路.
主要成果:
- Sln1作为流传感器,以压力依赖的方式定位到压力孔.
- 缺少Sln1的突变体表现出过度的流,超黑色化和非功能性压力.
- 通过Pka调节循环AMP (cAMP) 依赖的信号传输,Sln1与Pkc1通路并行运作.
- 这些通路调节压力和侵袭力产生.
结论:
- Sln1对于感知关键度值至关重要,确保正确的压力管功能和宿主透.
- Sln1和Pkc1信号通道共同调节压力和侵入力,这对引起爆发性疾病至关重要.
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