皮昂尼特林 A-D:由 Pseudomonas protegens 生产的化学天然产品
Emily Mevers1, Josep Saurí2, Eric J N Helfrich1
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School , 240 Longwood Avenue , Boston , Massachusetts 02115 , United States.
Journal of the American Chemical Society
|October 11, 2019
概括
研究人员发现了二种已知化合物之间独特的非酶反应产生的复杂代谢物 - - 氨酸. 这一发现突显了潜在的新抗真菌药物的新生物合成途径.
科学领域:
- 微生物学
- 自然产品化学
- 生物化学
背景情况:
- 细菌共生体提供化学防御,导致新的抗真菌药物和代谢物.
- 新型代谢物的发现通常依赖于识别独特的生物合成途径.
研究的目的:
- 发现和描述昆虫相关的Pseudomonas蛋白菌株的新代谢物.
- 阐明这些新代谢物的生物合成途径和化学结构.
主要方法:
- 在体内测试小鼠疹,以选细菌活动.
- 生物活性和质量导向分化用于代谢物分离.
- 基因组分析以确定生物合成基因集群.
主要成果:
- 发现了酸,一种高度缺乏的复杂芳香代谢物.
- 皮奥尼特林是由皮奥切林和皮罗尼特林生物合成中间体之间的自发非酶反应产生的.
- 特定的生物合成基因集群的识别,负责尼的生产.
结论:
- 酸是一种具有独特的化学结构和混合生物合成的新型天然产品.
- 尼生产中的非酶性步骤代表了一个意想不到的生物合成途径.
- 这一发现强调了仅仅依靠生物信息分析发现代谢物的局限性.
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