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哺乳动物细胞基因表达的超声波成像

Arash Farhadi¹, Gabrielle H Ho², Daniel P Sawyer¹

¹Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Science (New York, N.Y.)

|October 12, 2019

概括

研究人员开发了新的哺乳动物声学记者基因,用超声波可视化深层组织中的基因表达. 这一突破使得活体动物的细胞功能能够得到高分辨率的非侵入性成像.

科学领域:

生物医学成像
分子生物学
细胞成像

背景情况:

在生物深层组织中可视化细胞过程是一项挑战.
现有的方法缺乏与超声波对比相关的基因表达的非侵入性报告者.
超声波技术为生物医学成像提供高空间和时间分辨率.

研究的目的:

在哺乳动物细胞中设计基因编码的记者.
为了在深层组织中实现基因表达的非侵入性可视化.
开发一种用于高分辨率细胞成像的新工具.

主要方法:

从细菌基因集群中设计了一个真核生物遗传程序.
在哺乳动物细胞中引入基因程序.
用于超声波对比的表达细胞内充满空气的蛋白质纳米结构 (气囊).

主要成果:

已经成功地创造了哺乳动物的声学记者基因.
在体积密度低于0.5%的细胞的可视化.
证明了活动物基因表达的高分辨率成像.

结论:

哺乳动物声学记者基因提供了一种基于超声波的细胞成像的新方法.
这项技术允许对深层组织的基因表达进行非侵入性监测.
开发的报告器显著提升了体内细胞功能可视化.

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Cell Specific Gene Expression

Cell Specific Gene Expression

What is Gene Expression?

What is Gene Expression?

Overview
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Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...