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相关概念视频

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
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Accelerators

Accelerators in concrete serve as admixtures to speed up the hardening process, enabling the concrete to achieve early strength faster. Although accelerators do not necessarily impact the time it takes concrete to set, they reduce this time in practice. A common accelerator is calcium chloride, which is particularly useful for hastening early strength development in cold weather or for rapid repair jobs that require quick heat generation after mixing.
The effectiveness of calcium chloride can...

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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
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通过三重螺旋基终端固定增强亲和度的工程Aptamer

Lianhui Zhao1, Xiaoyan Qi1, Xiaochen Yan1

  • 1College of Food Science and Engineering , Ocean University of China , Qingdao , Shandong 266003 , China.

Journal of the American Chemical Society
|October 15, 2019
PubMed
概括

研究人员通过用三环结构固定它们的末端来设计出更稳定的体. 这提高了10倍的aptamer亲和力,并使葡萄酒中高灵敏的电化学aptassensor能够检测出lyszyme.

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科学领域:

  • 生物技术
  • 分子工程
  • 生物传感器的开发

背景情况:

  • 适应剂的灵活性往往会阻碍稳定的结构形成和最佳的结合.
  • 工程稳定性aptamer结构对于增强亲和力和传感器性能至关重要.

研究的目的:

  • 开发一个选择后的策略来稳定aptamer结构.
  • 为了增强抗溶酶胺的亲和力和性能.
  • 在复杂的矩阵中创建一个敏感的电化学感应器.

主要方法:

  • 使用长度优化的三螺旋结构稳定aptamer终端.
  • 通过这种后选择策略设计一种抗溶酶胺.
  • 开发使用DNA四面体间隔器的电化学感应器.

主要成果:

  • 改造后的阿普坦体对酶的亲和力增加了近10倍.
  • 与对照组相比,电化学感应器的检测极限降低了180倍.
  • 在真正的红葡萄酒样本中显示出高灵敏度和选择性.

结论:

  • 通过三螺旋结构的终端固定有效地稳定了体并增强了亲和力.
  • 在复杂的样本矩阵中检测分析物具有显著的潜力.
  • 这一战略为aptamer工程和生物传感器应用提供了有前途的方法.