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相关概念视频

Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Operon Model01:23

Operon Model

The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA (thiogalactoside...

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相关实验视频

Updated: Jun 18, 2026

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
11:49

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

Published on: August 21, 2018

兰巴达抑制器突变增加了操作者结合的亲和力和特异性.

H C Nelson, R T Sauer

    Cell
    |September 1, 1985
    PubMed
    概括
    此摘要是机器生成的。

    第二位逆转发现了突变,增强了兰巴抑制剂的DNA结合亲和力和特异性. 这些遗传修饰改善了抑制剂-DNA相互作用,为蛋白质-DNA结合机制提供了洞察力.

    更多相关视频

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

    Published on: March 29, 2019

    An Assay for Quantifying Protein-RNA Binding in Bacteria
    07:02

    An Assay for Quantifying Protein-RNA Binding in Bacteria

    Published on: June 12, 2019

    相关实验视频

    Last Updated: Jun 18, 2026

    A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
    11:49

    A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

    Published on: August 21, 2018

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
    08:54

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

    Published on: March 29, 2019

    An Assay for Quantifying Protein-RNA Binding in Bacteria
    07:02

    An Assay for Quantifying Protein-RNA Binding in Bacteria

    Published on: June 12, 2019

    科学领域:

    • 分子生物学分子生物学
    • 遗传学 是一个遗传学.
    • 生物化学 生物化学

    背景情况:

    • 兰巴达抑制蛋白调节病毒DNA复制.
    • 了解蛋白质-DNA相互作用对于分子生物学至关重要.
    • 第二部位逆转是一种研究蛋白质功能的方法.

    研究的目的:

    • 识别氨基酸替代物,以提高兰巴抑制剂与操作者DNA的结合亲和力和特异性.
    • 为了研究增加压缩器-操作器相互作用的结构基础.

    主要方法:

    • 采用了内部的,第二站点逆转突变的突变发生.
    • 用替代品进行净化抑制剂的分析,以检测DNA结合.
    • 测量了结合动力学 (结合和解离率).

    主要成果:

    • 与野生类型相比,第二位置换增加了抑制剂-运营者DNA结合亲和力3-600倍.
    • 亲密关系的增强是由于更快的关联率和更慢的分离率.
    • 阿尔法2和阿尔法3螺旋体中的替代物与DNA骨干形成了新的键.
    • 在α5螺旋的替换间接增加了操作者亲和力.

    结论:

    • 第二部位逆转在识别蛋白质-DNA结合的有益突变方面是有效的.
    • 特定的氨基酸替代可以通过直接或间接的机制显著增强抑制剂-运营者相互作用.
    • 结构洞察力表明,有针对性的修改可以优化蛋白质-DNA结合亲和力和特异性.