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相关概念视频

Positive Regulator Molecules01:45

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To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
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Circadian Rhythms and Gene Regulation02:19

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The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
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Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
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M-Cdk Drives Transition Into Mitosis02:15

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Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
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Separation of Sister Chromatids02:17

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At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
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Anaphase Promoting Complex00:50

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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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相关实验视频

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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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与MEL细胞承诺与cordycepin的同步.

R Levenson, J Kernen, D Housman

    Cell
    |December 1, 1979
    PubMed
    概括

    作为一种核酸模拟物,cordicepin 阻断了小鼠红血球血 (MEL) 细胞中的红色球体分化. 这一发现揭示了一种新的分子机制,控制细胞对终端分化的承诺.

    科学领域:

    • * 分子生物学 * 分子生物学
    • * 细胞分化的过程
    • * 癌症研究研究

    背景情况:

    • *小鼠红血球白血病 (MEL) 细胞是研究红细胞分化的一个模型系统.
    • * 致力于终端分化涉及复杂的分子事件.
    • *核酸类似物可以调节细胞过程.

    研究的目的:

    • * 为了研究cordycepin对MEL细胞分化的影响.
    • * 确定cordycepin在红状腺结合的分子事件中的作用.
    • * 探索cordycepin在特定阶段阻止分化的潜力.

    主要方法:

    • * 用不同剂量的皮质cepin治疗分化的MEL细胞.
    • *细胞结合和细胞毒性的评估.
    • *在诱导剂的存在下,用cordycepin进行逆转实验.

    主要成果:

    • * 科尔迪塞宾迅速抑制了MEL细胞对红状腺分化的承诺.
    • * 在非细胞毒性剂量时发生抑制.
    • * 康迪塞宾治疗的逆转允许快速和同步的细胞承诺.

    结论:

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    • * Cordycepin揭示了以前未被识别的红状腺分化承诺的方面.
    • * MEL细胞可以被cordycepin.commit之前就被阻止.
    • * Cordycepin提供了一个研究细胞命运决定的精确分子定时的工具.