对于细菌检测,需要NOD1和NOD2的棕化
Yan Lu1,2, Yuping Zheng1, Étienne Coyaud3
1Department of Cell Biology, and Department of Pathology Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
核酸寡合化域 (NOD) 类受体1和2 (NOD1/2) 需要S-palmitoylation用于膜招募和免疫信号. 这种由ZDHHC5介导的修饰对于对细菌糖的反应至关重要.
科学领域:
- 免疫学
- 细胞生物学
- 分子生物学
背景情况:
- 核酸寡合化域 (NOD) 类受体1和2 (NOD1/2) 是细胞内模式识别蛋白.
- 对于对微生物糖的免疫反应,NOD1/ 2的激活是必不可少的.
- 膜招募对于NOD1/2信号至关重要,缺陷与炎症性疾病有关.
研究的目的:
- 研究NOD1/2在细胞内膜中的分子机制.
- 确定涉及NOD1/2膜局部化的翻译后修饰和酶.
- 确定NOD1/2膜招募在免疫信号中的功能意义.
主要方法:
- 研究了S-palmitoylation在NOD1/2膜招募和信号中的作用.
- 通过生物化学测试确定了对NOD1/2 S-palmitoylation负责的棕转移酶.
- 分析了与疾病相关的NOD2突变以检测S-palmitoylation中的缺陷.
主要成果:
- 发现NOD1/ 2的S- 棕化对它们在细胞内膜的吸收至关重要.
- ZDHHC5被确定为特定的棕转移酶,可催化NOD1/2 S-棕转化.
- 一些与炎症相关的NOD2突变影响了S-palmitoylation.
结论:
- ZDHHC5-介导的S-palmitoylation是NOD1/2功能的一个关键的翻译后修饰.
- 这种修饰是适当的膜局部化,糖体识别和有效的免疫反应所必需的.
- 缺陷的NOD1/2S-palmitoylation可能导致炎症性疾病.
更多相关视频
14:25Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Published on: December 12, 2017
11:50Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
Published on: October 1, 2015
相关概念视频
Bacterial Signaling
Mechanism of Lamellipodia Formation
Cell Signaling in Plants
Notch Signaling Pathway
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
Mechanism of Filopodia Formation
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Tail-anchoring of Proteins in the ER Membrane
