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单细胞测序小鼠心脏免疫透在压力过载驱动的心脏衰竭揭示免疫激活的程度

Elisa Martini¹, Paolo Kunderfranco², Clelia Peano^3,4

¹Adaptive Immunity Laboratory (E.M., M.C., M.K.), Humanitas Clinical and Research Center, Rozzano, Milan, Italy.

|October 31, 2019

概括

除了巨细胞和T细胞,心力衰竭还涉及各种免疫细胞,包括中性细胞,B细胞,NK细胞和巨细胞. 它们的激活和特定分子为心免疫学提供了新的诊断和治疗点.

科学领域:

心血管疾病研究
免疫学
单细胞基因组学

背景情况:

炎症是心脏病进展的关键因素,其中巨细胞和T淋巴细胞起着关键作用.
对于其他免疫细胞子集有助于心脏毒性的理解有限.

研究的目的:

在压力过载心力衰竭的小鼠模型中全面绘制心脏免疫细胞组成.
确定不同疾病阶段参与心脏毒性反应的免疫细胞子集.

主要方法:

在健康和心力衰竭模型中对心脏CD45+细胞进行单细胞RNA测序.
与多参数流细胞计,免疫组织化学和组织澄清免疫光学进行整合.
对老鼠和人类心脏组织样本的分析.

主要成果:

所有主要的免疫细胞亚群 (巨细胞,B细胞,T细胞,NK细胞,中性粒细胞,巨细胞) 都存在于健康和患病的心脏中.
压力过重会诱导各种细胞类型的免疫激活,包括中性粒细胞,B细胞,NK细胞和乳腺细胞.
分组特异性分子 (例如,上素M,PD-1) 被上调,这可能解释了临床观察.

结论:

心力衰竭的发病涉及比以前更广泛的免疫细胞.
研究这些免疫子集为心脏免疫学研究提供了新的途径.
特定子集的分子代表了潜在的心力衰竭诊断或治疗点.

关键词:

心脏衰竭对心脏有毒性血压性心力衰竭在COSTATIN M 编程细胞死亡,I型序列分析,RNA

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