在Xenopus蛋提取物中自发出现细胞样组织
Xianrui Cheng1, James E Ferrell1,2
1Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305-5174, USA. xianrui.cheng@gmail.com james.ferrell@stanford.edu.
Science (New York, N.Y.)
|November 2, 2019
概括
细胞质可以自组织成类似细胞的区间,显示出新的空间组织. 这一对于早期胚胎发育至关重要的过程需要ATP和微管聚合.
科学领域:
- 细胞生物学
- 发育生物学
- 生物物理
背景情况:
- 细胞遗传包括遗传物质和有组织的细胞组成部分.
- 在细胞形成中,de novo自我组织与遗传组织的作用尚不清楚.
研究的目的:
- 研究细胞质的自我组织成功能性细胞样结构的能力.
- 确定细胞质自我组织和细胞周期进展的要求.
主要方法:
- 使用了Xenopus laevis的蛋提取物.
- 诱导同质化细胞质的自我组织.
- 在循环提取物中观察到分区形成和细胞分裂周期.
主要成果:
- 在约30分钟内,同质化细胞质自组织成类似细胞的部分 (300-400μm).
- 需要三酸腺和微管聚合物形成.
- 使用精子循环提取物证明了分离和重组,模仿了线粒循环.
结论:
- 细胞质具有新的空间组织的内在能力.
- 细胞质的自我组织可以产生早期胚胎发育的必要空间组织和细胞循环功能.
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