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使用长期体外胚胎培养对灵长类动物植入后早期发育进行剖析
Yuyu Niu1, Nianqin Sun2, Chang Li2
1Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. tant@lpbr.cn.com niuyy@lpbr.cn wji@lpbr.cn belmonte@salk.edu.
Science (New York, N.Y.)
|November 2, 2019
概括
研究人员开发了一种新型的体外培养系统,用于子胚胎,使得早期灵长类动物发育的研究成为可能,并揭示了胃化过程中的关键细胞和分子事件.
科学领域:
- 发育生物学
- 灵长类动物的胚胎形成
- 干细胞生物学
背景情况:
- 了解早期哺乳动物的发育,特别是从周植入到胃形成的过渡,对于理解身体图的形成至关重要.
- 从历史上看,技术和伦理障碍限制了灵长类动物的早期胚胎阶段的研究.
研究的目的:
- 建立一个强大的体外培养系统用于Cynomolgus子胚胎.
- 研究早期植入后灵长类发展的细胞和分子机制.
主要方法:
- 开发一个扩展的Cynomolgus子胚胎体外培养系统 (长达20天).
- 单细胞RNA测序 (scRNA-seq) 用于分析血统发展轨迹.
- 使用测序 (scATAC-seq) 确定关键转录因子的单细胞测定.
主要成果:
- 培养的子胚胎成功地重现了关键的发育里程碑,包括血统分离和原始生殖细胞样细胞 (PGCLC) 的分化.
- scRNA-seq揭示了原始内皮,热皮,表皮细胞和PGCLC的不同发育途径.
- 在此期间,scATAC- seq 确定了对细胞类型规范至关重要的特定转录因子.
结论:
- 已建立的培养系统为非人类灵长类胚胎形成提供了前所未有的窗口.
- 这项研究阐明了早期植入后发育过程中的关键细胞和分子事件,为人类发育提供了潜在的见解.
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Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
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