用粘合性细菌纳米纤维蛋白ATAA覆盖的人造细胞的自下而上的创建
Kosaku Noba1, Masahito Ishikawa1, Atsuko Uyeda2
1Department of Biomolecular Engineering, Graduate School of Engineering , Nagoya University , Furo-cho, Chikusa-ku , Nagoya 464-8603 , Japan.
Journal of the American Chemical Society
|November 8, 2019
概括
研究人员制造了一种仿细胞脂质体, 这种新型系统成功地模仿细菌细胞表面功能,并粘附于表面,使新的生物技术应用成为可能.
科学领域:
- 合成生物学和生物材料科学
- 细菌细胞表面工程和蛋白质复合.
背景情况:
- 细菌细胞表面结构对于细胞功能至关重要.
- 对重建细菌细胞表面结构的研究有限.
研究的目的:
- 在底部向上创建一个细胞大小的脂质体,覆盖着粘合性细菌纳米纤维蛋白 AtaA.
- 在不使用本地蛋白质分泌和组装机械的情况下实现这一目标.
主要方法:
- 脂质体是用修改后的脂体进行的.
- 在大肠杆菌中表达了与SNAP标签融合的截断的AtaA蛋白.
- 脂质体被AtaA融合蛋白所装饰.
主要成果:
- 复制的脂质体表现出与本地Acinetobacter sp相似的表面结构和功能. 托尔是5个细胞.
- 人工细胞附着在恐水和爱水表面.
- 该系统可以在粘合状态下驱动酶反应.
结论:
- 开发的人工细胞系统使得在模仿细胞的环境中研究细胞表面蛋白质.
- 用ATAA装饰的人造细胞为需要表面固定的生物技术应用提供了潜力,特别是在转基因生物被限制的地方.
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