生物对等的异二氧化结合
Rebecca J B Schäfer1, Mattia R Monaco1, Mao Li1
1Laboratory of Organic Chemistry , ETH Zurich, D-CHAB , Vladimir-Prelog-Weg 3 , Zurich 8093 , Switzerland.
Journal of the American Chemical Society
|November 12, 2019
概括
在水性环境中提供快速,选择性的标签. 这种方法与细胞系统兼容,与其他生物结合技术正交.
科学领域:
- 化学生物学
- 有机化学
- 生物结合化学
背景情况:
- 在复杂的生物系统中选择性标记和成像生物分子的过程中,生物直角反应至关重要.
- 现有的方法通常面临速度,选择性或与水环境的兼容性方面的限制.
研究的目的:
- 介绍和描述异和氧化物之间的新型结合反应,用于生物对等应用.
- 证明这种结合在细胞成像中的实用性及其对已确定的生物正交反应的正交性.
主要方法:
- 在水溶液中研究了异二结合的动力学和化学选择性.
- 应用结合图像代谢标记的细胞表面甘.
- 进行活细胞双标记实验,以评估应变促进的亚酸循环添加 (SPAAC).
主要成果:
- 在水中具有很高的化学选择性,异酸结合过程迅速进行 (k ≈ 1 M-1 s-1).
- 反应表明对细胞环境中的各种功能组的耐受性.
- 双标记实验证实了活细胞中这种SPAAC结合的正交性.
结论:
- 异二结合是一种强大而高效的新工具,用于生物对角化学.
- 它的速度,选择性和直角性使其适用于先进的细胞成像和标签应用.
相关概念视频
02:34Structural Isomerism
21.4K
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
21.4K
03:31Labeling DNA Probes
9.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.1K