海洋蛋白质细菌通过β-酸循环代谢糖
Lennart Schada von Borzyskowski1, Francesca Severi2, Karen Krüger3
1Department of Biochemistry & Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany. schada@mpi-marburg.mpg.de.
Nature
|November 15, 2019
概括
海洋细菌通过β-酸循环 (BHAC) 吸收海洋糖酸盐. 这种有效的途径涉及关键的酶,如新型的胺还原酶,在全球分布,对碳循环至关重要.
科学领域:
- 海洋微生物学
- 生物地化学循环
- 代谢途径
背景情况:
- 糖是一种主要的海洋有机碳来源,来自植物.
- 海洋细菌中的糖酸盐代谢物的命运尚不清楚.
- 之前的知识缺乏有效的glyoxylate同化途径.
研究的目的:
- 阐明海洋细菌中氧酸盐同化过程的生物化学路径.
- 确定参与该途径的关键酶的结构.
- 评估这种途径在海洋环境中的流行和活动.
主要方法:
- β-酸循环的生化特征 (BHAC).
- 对BHAC酶的结构分析,包括一种新型的胺还原酶.
- 海洋元基因组分析以确定BHAC的分布和丰度.
- 在植物浮游生物开花期间进行实地研究,以测量糖摄取和BHAC基因表达.
主要成果:
- 海洋蛋白质细菌通过BHAC同化酸盐,这是一个四步制造酸盐的途径.
- BHAC是已知的最有效的氧酸同化途径.
- BHAC 在全球分布,比糖酸盐通路多20倍.
- 在植物浮游生物的开花期间,BHAC基因被积极转录,这表明了显著的糖同化.
结论:
- BHAC是海洋细菌利用浮游生物衍生糖的关键途径.
- 这一途径代表了植物浮游生物和细菌浮游生物之间的显著,以前未被描述的食物链接.
- 在海洋碳循环中, BHAC 的效率和丰富性凸显了它的重要性.
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