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相关概念视频

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One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
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Secondary Active Transport01:32

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One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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相关实验视频

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Single-Molecule Imaging of Nuclear Transport
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用单分子分辨率量化二次运输

Gabriel A Fitzgerald1, Daniel S Terry1,2, Audrey L Warren3

  • 1Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.

Nature
|November 15, 2019
PubMed
概括

研究人员可视化了单个的二次活性传送器,MHST,以了解它们的传输机制. 他们发现传递器活动取决于导向和基质,揭示了神经递质:同传递器功能的洞察.

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科学领域:

  • 生物化学
  • 分子生物学
  • 生物物理

背景情况:

  • 二次活性运输体对于生理过程至关重要,它们利用离子梯度进行膜运输.
  • 由于速度缓慢和组合方法的局限性,调查传送机制具有挑战性.

研究的目的:

  • 为了量化单个MhsT输送物的活性,用于神经递质:共导体的模型.
  • 在单个分子水平上阐明基质运输机制.

主要方法:

  • 通过脂质双层成像个体MhsT传送器活动.
  • 用单个和多个转换分辨率量化运输.
  • 分析基板的运输动态.

主要成果:

  • 它的运输活性取决于它的生理方向.
  • 运输周期中的速度限制步骤因运输的基板而异.
  • 证据表明细胞外结位会影响运输动力学.

结论:

  • 单分子成像为研究传送机制提供了前所未有的分辨率.
  • MhsT的运输周期由基板结合和传送器方向调节.
  • 这些发现提供了神经递质:共导体的功能动态.