通过冷EM揭示的头对头MCM双六合体形成的机制

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Thomas C R Miller1, Julia Locke1, Julia F Greiwe1

  • 1Macromolecular Machines Laboratory, Francis Crick Institute, London, UK.

Nature
|November 22, 2019
PubMed
概括

原始识别复合体 (ORC) 为DNA复制加载两个MCM环. 这项研究揭示了一种合加载机制,其中第一个螺旋酶促进了第二个螺旋酶的招募,使以前的模型相协调.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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